GNAS Antibody

Product Specifications

Product Name Alternative

GNAS, GNAS complex locus, AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, NESP, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3, OTTHUMP00000031742, OTTHUMP00000196026, OTTHUMP00000196030, adenylate cyclase-stimulating G alpha protein, guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1, guanine nucleotide regulatory protein, neuroendocrine secretory protein, secretogranin VI

UniProt

O95467

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-QAARSNSDGEKATK

Target

GNAS

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 45.7kDa according to NP_000507.1) . Recommended concentration: 1-3μg/mL. Approx. 50kDa band observed in lysates of Mouse and Rat Brain (calculated MW of 45.6kDa according to NP_963910.1) . Recommended concentration: 0.1-0.3μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001070956.1, NP_001070957.1, NP_000507.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.