Npc1 Antibody
Npc1 Antibody
Product Specifications
Product Name Alternative
Npc1, Niemann Pick type C1, A430089E03Rik, C85354, D18Ertd139e, D18Ertd723e, lcsd, nmf164, spm, niemann-Pick C1 protein, sphingomyelinosis
UniProt
O35604
Immunogen
The immunogen for this antibody is: C-TYHTILKTSADYTD
Target
Npc1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience, Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 142kDa according to NP_032746.2. The 36kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Mouse
Tested Applications
ELISA
NCBI Accession Number
NP_032746.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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