CIRBP Antibody

Product Specifications

Product Name Alternative

CIRBP, cold inducible RNA binding protein, CIRP, Cold-inducible RNA-binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein

UniProt

Q14011

Reactivity

Mouse, Rat

Immunogen

The immunogen for this antibody is: C-QAGKSSDNRSR

Target

CIRBP

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 18kDa band observed in Mouse and Rat Testis lysates (calculated MW of 18.6kDa according to Mouse NP_031731.1 and Rat NP_112409.2) . Recommended concentration: 0.05-0.2μg/mL. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Canine, Human

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001271.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.