IGF1 Antibody

Product Specifications

Product Name Alternative

IGF1, insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

UniProt

P05019

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RSVRAQRHTD

Target

IGF1

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1) . Recommended concentration 0.5-2μg/mL. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

NCBI Accession Number

NP_001104753.1, NP_000609.1, NP_001104755.1, NP_001104754.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.