BIRC6 Antibody
BIRC6 Antibody
Product Specifications
Product Name Alternative
BIRC6, baculoviral IAP repeat-containing 6, APOLLON, BRUCE, FLJ13726, FLJ13786, KIAA1289, ubiquitin-conjugating BIR-domain enzyme apollon
UniProt
Q9NR09
Immunogen
The immunogen for this antibody is: C-SREYDGNIRQAT
Target
BIRC6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 90kDa and 70kDa in lysates of cell lines A431 and HeLa after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 530kDa according to NP_057336.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Human, Mouse, Rat
Tested Applications
ELISA
NCBI Accession Number
NP_057336.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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