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LIMS2 Antibody

LIMS2 Antibody

Product Specifications

Product Name Alternative

LIMS2, LIM and senescent cell antigen-like domains 2, FLJ10044, PINCH2, ILK-binding protein, OTTHUMP00000203848, OTTHUMP00000203850, OTTHUMP00000203866

UniProt

Q7Z4I7

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RWSNMSDALA

Target

LIMS2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology, Epigenetics & Chromatin, Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75μg/mL.

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_001129509.2, NP_001154875.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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