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ZDHHC1 Antibody

ZDHHC1 Antibody

Product Specifications

Product Name Alternative

ZDHHC1, zinc finger, DHHC-type containing 1, C16orf1, HSU90653, ZNF377, DHHC-domain-containing cysteine-rich protein, zinc finger, DHHC domain containing 1

UniProt

Q8WTX9

Immunogen

The immunogen for this antibody is: C-RNYRLFLHS

Target

ZDHHC1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 20kDa in lysates of cell line HEK293 after 2μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 54.8kDa according to NP_037436.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Human, Mouse, Rat

Tested Applications

ELISA

NCBI Accession Number

NP_037436.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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