NOX5 Antibody
NOX5 Antibody
Product Specifications
Product Name Alternative
NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444
UniProt
Q96PH1
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-EWHPFTISSAPEQKD
Target
NOX5
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3) . Recommended concentration: 0.5-2μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_001171708.1, NP_078781.3, NP_001171709.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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