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SRGAP2 Antibody

SRGAP2 Antibody

Product Specifications

Product Name Alternative

SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2

UniProt

O75044

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-KQEDRQTPRSPDST

Target

SRGAP2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional) . Recommended concentration: 0.1-0.3μg/mL.Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF) .

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_056141.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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