PLA2G4F Antibody

Product Specifications

Product Name Alternative

PLA2G4F, phospholipase A2, group IVF, DKFZp666G192, DKFZp781B229, PLA2G4FZ, cytosolic phospholipase A2 zeta, cPLA2-zeta

UniProt

Q68DD2

Immunogen

The immunogen for this antibody is: C-QTAEEKAFGDFVINR

Target

PLA2G4F

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Human, Mouse, Rat

Tested Applications

ELISA

NCBI Accession Number

NP_998765.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.