DSP Antibody
DSP Antibody
Product Specifications
Product Name Alternative
DSP, desmoplakin, DPI, 250kDa paraneoplastic pemphigus antigen, desmoplakin I
UniProt
P15924
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QESVKQAHLRNEH
Target
DSP
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1μg/mL) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_004406.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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