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SOX11 Antibody

SOX11 Antibody

Product Specifications

Product Name Alternative

SOX11, SRY (sex determining region Y) -box 11, SRY (sex-determining region Y) -box 11, SRY-related HMG-box gene 11, transcription factor SOX-11

UniProt

P35716

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SRLYYSFKNITKQHP

Target

SOX11

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 65 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 65kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 46.7kDa according to NP_003099.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Dictor et al, Haematologica. 2009 Nov;94 (11) :1563-8. PMID: 19880779) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3, Mouse and Rat Brain for this particular batch.

Prediction Reactivity

Bovine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_003099.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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