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PI15 Antibody

PI15 Antibody

Product Specifications

Product Name Alternative

PI15, peptidase inhibitor 15, CRISP8, DKFZp686F0366, P24TI, P25TI, 25 kDa trypsin inhibitor, CRISP-8, PI-15, cysteine-rich secretory protein 8, protease inhibitor 15, sugarCrisp

UniProt

O43692

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RTGRYRSILQLVKP

Target

PI15

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 33 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 33kDa band observed in lysates of cell line HeLa (calculated MW of 29.1kDa according to NP_056970.1) . Recommended concentration: 0.3-1μg/mL. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_056970.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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