PI15 Antibody
PI15 Antibody
Product Specifications
Product Name Alternative
PI15, peptidase inhibitor 15, CRISP8, DKFZp686F0366, P24TI, P25TI, 25 kDa trypsin inhibitor, CRISP-8, PI-15, cysteine-rich secretory protein 8, protease inhibitor 15, sugarCrisp
UniProt
O43692
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RTGRYRSILQLVKP
Target
PI15
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 33 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 33kDa band observed in lysates of cell line HeLa (calculated MW of 29.1kDa according to NP_056970.1) . Recommended concentration: 0.3-1μg/mL. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_056970.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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