ZAP70 Antibody

Product Specifications

Product Name Alternative

ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD

UniProt

P43403

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SDGYTPEPARITSPD

Target

ZAP70

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 70 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2) . Recommended concentration: 0.3-1μg/mL.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer) . Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IP, WB

NCBI Accession Number

NP_001070.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.