P2rx4 Antibody
P2rx4 Antibody
Product Specifications
Product Name Alternative
P2rx4, purinergic receptor P2X, ligand-gated ion channel, 4, P2X4, AI504491, AW555605, D5Ertd444e, ATP receptor, OTTMUSP00000023741, OTTMUSP00000023745, OTTMUSP00000023746, OTTMUSP00000023747, P2X purinoceptor 4, ionotropic purinergic receptor
UniProt
Q99571
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-ETDSVVSSVTTKAK
Target
P2rx4
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 60 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_035156.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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