BRWD1 Antibody

Product Specifications

Product Name Alternative

BRWD1, bromodomain and WD repeat domain containing 1, C21orf107, FLJ43918, N143, WDR9, OTTHUMP00000068796, OTTHUMP00000068797, OTTHUMP00000068928, OTTHUMP00000207967, WD repeat protein WDR9-form2, WD repeat-containing protein 9, bromodomain and WD repeat-containing protein 1, transcriptional unit N143

UniProt

Q9NSI6

Immunogen

The immunogen for this antibody is: RQNCKGDSQPNKSIR

Target

BRWD1

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 100 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 100kDa band in Human Heart and Skeletal Muscle lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 263kDa according to NP_061836.2. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We found identical results with an antibody generated to a different part of the protein (EB11491) .

Prediction Reactivity

Human

Tested Applications

ELISA

NCBI Accession Number

NP_387505.1, NP_001007247.1, NP_061836.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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