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UBN1 Antibody

UBN1 Antibody

Product Specifications

Product Name Alternative

UBN1, ubinuclein 1, VT, VT4, HIRA-binding protein, OTTHUMP00000159925, OTTHUMP00000159926, ubinuclein-1, ubiquitously expressed nuclear protein

UniProt

Q9NPG3

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-DNSEAYDELVPASLT

Target

UBN1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 120 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 120kDa band observed in lysates of cell line MOLT4 (calculated MW of 122kDa according to NP_058632.2) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat, Zebrafish

Tested Applications

ELISA, WB

NCBI Accession Number

NP_058632.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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