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Nsg1 Antibody

Nsg1 Antibody

Product Specifications

Product Name Alternative

Nsg1, neuron specific gene family member 1, Bsmrb, Neep21, PEP1, brain neuron cytoplasmic protein 1/2, brain specific mRNA b, neuron-specific protein family member 1

UniProt

P42857

Reactivity

Mouse, Rat

Immunogen

The immunogen for this antibody is: EKGTKQPLLEDGFD-C

Target

Nsg1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 22 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of EB11637 (red) in the neurons only. Recommended concentration: 1-5μg/mL.

Prediction Reactivity

Bovine, Human

Tested Applications

ELISA, ICC, WB

NCBI Accession Number

NP_077042.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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