PCNA Antibody
PCNA Antibody
Product Specifications
Product Name Alternative
PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin
UniProt
P12004
Reactivity
Human, Mouse, Porcine, Rat
Immunogen
The immunogen for this antibody is: C-EAPNQEKVSDYE
Target
PCNA
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 30 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1) . Approx.35kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33kDa in Pig Spleen lysates. (calculated MW of 28.8kDa according to Mouse NP_035175.1, Rat NP_071776.1 and Pig NP_001278854.1) .The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186 (5) :645-54.) . Recommended concentration: 0.03-0.1μg/mL.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as reported in combination with EB11650. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine
Tested Applications
EIA, ELISA, WB
NCBI Accession Number
NP_002583.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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