C3 Antibody
C3 Antibody
Product Specifications
Product Name Alternative
C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3
UniProt
P01024
Reactivity
Human
Immunogen
The immunogen for this antibody is: QQRTFISPIKCRE
Target
C3
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 125 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to the largest fragment C3 alpha chain (aa672-1663) . Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Testis. Recommended concentration: 5μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_000055.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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