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EHD2 Antibody

EHD2 Antibody

Product Specifications

Product Name Alternative

EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617

UniProt

Q9NZN4

Reactivity

Human

Immunogen

The immunogen for this antibody is: CRLVPPSKRRHKGSA

Target

EHD2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunofluorescence: strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF, WB

NCBI Accession Number

NP_055416.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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