EHD2 Antibody
EHD2 Antibody
Product Specifications
Product Name Alternative
EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
UniProt
Q9NZN4
Reactivity
Human
Immunogen
The immunogen for this antibody is: CRLVPPSKRRHKGSA
Target
EHD2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunofluorescence: strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF, WB
NCBI Accession Number
NP_055416.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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