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PARP12 Antibody

PARP12 Antibody

Product Specifications

Product Name Alternative

PARP12, poly (ADP-ribose) polymerase family, member 12, HGNC:21919, FLJ22693, PARP-12, ZC3H1, ZC3HDC1, zinc finger CCCH type domain containing 1, zinc finger CCCH-type domain containing 1

UniProt

Q9H0J9

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-NAHDIKNKSSAP

Target

PARP12

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunofluorescence: Anonymous customer has confirmed specificity by siRNA-mediated PARP2 knockdown.

Prediction Reactivity

Canine

Tested Applications

ELISA, IF

NCBI Accession Number

NP_073587.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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