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ACAT1 Antibody

ACAT1 Antibody

Product Specifications

Product Name Alternative

ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase

UniProt

P24752

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-DEEYKRVDFSKVPK

Target

ACAT1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 45kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1 and 44.81kDa to Mouse NP_659033.1) . Recommended concentration: 0.01-0.03μg/mL. Primary incubation was 1 hour.Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa. This data is from a previous batch, not on sale.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_000010.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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