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ICAM1 Antibody

ICAM1 Antibody

Product Specifications

Product Name Alternative

ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

UniProt

P05362

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: SNCPDGQSTAKT

Target

ICAM1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 100kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 100kDa band observed in lysates of cell lines Daudi, HeLa and NIH3T3 (calculated MW of 57.8kDa according to Human NP_000192.2 and 58.8kDa according to Mouse NP_034623.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138 (5) :837-47. PMID: 19661147), and other commercial sources. Recommended concentration: 1-3μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membrane of NIH3T3 cells. Recommended concentration: 5μg/mL.

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_000192.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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