What is the catalog number for Calbindin D28 Antibody?

The catalog number for Calbindin D28 Antibody is orb1248565.

What sizes are available for Calbindin D28 Antibody?

Calbindin D28 Antibody is available in the following size(s): 0.1 mg.

Is Calbindin D28 Antibody in stock?

Calbindin D28 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Calbindin D28 Antibody

Product Specifications

Product Name Alternative

CALB1, calbindin 1, 28kDa, CALB, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, calbindin, calbindin 1, (28kD), calbindin D28, vitamin D-dependent calcium-binding protein, avian-type

UniProt

P05937

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-KTFVDQYGQRDDGK

Target

Calbindin D28

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 26kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170 (1-2) :207-15. PMID: 3691519. Recommended concentration: 0.03-0.1μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_004920.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.