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Hsp31p Antibody

Hsp31p Antibody

Product Specifications

Product Name Alternative

Hsp31p, YDR533C

UniProt

Q04432

Reactivity

Yeast

Immunogen

The immunogen for this antibody is: C-ETGKFGWDEHSLAKD

Target

Hsp31p

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 24kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1) . Recommended concentration: 0.02-0.06μg/mL. This antibody has been successfully used in the following paper: Natkańska et al (2018) PMID: 29264711.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_010822.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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