Hsp31p Antibody
Hsp31p Antibody
Product Specifications
Product Name Alternative
Hsp31p, YDR533C
UniProt
Q04432
Reactivity
Yeast
Immunogen
The immunogen for this antibody is: C-ETGKFGWDEHSLAKD
Target
Hsp31p
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 24kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1) . Recommended concentration: 0.02-0.06μg/mL. This antibody has been successfully used in the following paper: Natkańska et al (2018) PMID: 29264711.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_010822.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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