EAAT1/GLAST1 Antibody
EAAT1/GLAST1 Antibody
Product Specifications
Product Name Alternative
SLC1A3, solute carrier family 1 (glial high affinity glutamate transporter), member 3, EA6, EAAT1, FLJ25094, GLAST, GLAST1, GLAST-1, OTTHUMP00000116002, excitatory amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 1, solute carrier family 1 member 3
UniProt
P43003
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RSFKVPIQANET
Target
EAAT1 / GLAST1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 60kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3) . Recommended concentration: 0.1-0.3μg/mL. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_004163.3, NP_001160167.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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