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TNNI3 Antibody

TNNI3 Antibody

Product Specifications

Product Name Alternative

TNNI3, troponin I type 3 (cardiac), CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle

UniProt

P19429

Reactivity

Human, Mouse, Porcine, Rat

Immunogen

The immunogen for this antibody is: C-KVTKNITEIAD

Target

TNNI3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 27 + 28kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 25+26kDa doublet band observed in Human Heart, approx 24+27kDa in Mouse Heart, approx 25kDa in Rat Heart and approx 27kDa in Pig Heart lysates (calculated MW of 24kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3kDa according to Mouse NP_033432.1 and Ra tNP_058840.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_000354.4

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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