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HIC1 Antibody

HIC1 Antibody

Product Specifications

Product Name Alternative

HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, hic-1, hypermethylated in cancer 1 protein, zinc finger and BTB domain-containing protein 29

UniProt

Q14526

Reactivity

Human

Immunogen

The immunogen for this antibody is: NTHCAELYASGP

Target

HIC1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1μg/mL.

Prediction Reactivity

Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_006488.2, NP_001091672.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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