HIC1 Antibody
HIC1 Antibody
Product Specifications
Product Name Alternative
HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, hic-1, hypermethylated in cancer 1 protein, zinc finger and BTB domain-containing protein 29
UniProt
Q14526
Reactivity
Human
Immunogen
The immunogen for this antibody is: NTHCAELYASGP
Target
HIC1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1μg/mL.
Prediction Reactivity
Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_006488.2, NP_001091672.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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