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RPA1/RPA70 Antibody

RPA1/RPA70 Antibody

Product Specifications

Product Name Alternative

RPA1, replication protein A1, 70kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein

UniProt

P27694

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: CKSYEDATKITVRSN

Target

RPA1 / RPA70

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 70 + 80kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1) . The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of Mouse Lymph Node. Recommended concentration: 0.3-1μg/mL.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_002936.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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