RPA1/RPA70 Antibody
RPA1/RPA70 Antibody
Product Specifications
Product Name Alternative
RPA1, replication protein A1, 70kDa, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, single-stranded DNA-binding protein
UniProt
P27694
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: CKSYEDATKITVRSN
Target
RPA1 / RPA70
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 70 + 80kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1) . The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of Mouse Lymph Node. Recommended concentration: 0.3-1μg/mL.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_002936.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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