CREB1 Antibody
CREB1 Antibody
Product Specifications
Product Name Alternative
CREB1, cAMP responsive element binding protein 1, CREB, CREB-1, active transcription factor CREB, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, cyclic AMP-responsive element-binding protein 1, transactivator protein
UniProt
P16220
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-DSQESVDSVTDSQK
Target
CREB1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 45kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, WB
NCBI Accession Number
NP_004370.1, NP_604391.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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