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Prdm9 Antibody

Prdm9 Antibody

Product Specifications

Product Name Alternative

Prdm9, PR domain containing 9, RP23-64O5.3, BC012016, Dsbc1, G1-419-29, Meisetz, Rcr1, repro7, PR domain zinc finger protein 9, PR domain-containing protein 9, PR-domain zinc finger protein, VPR domain containing 9, histone-lysine N-methyltransferase PRDM9, hybrid sterility protein 1, meiosis-induced factor containing a PR/SET domain and zinc-finger motif

UniProt

Q96EQ9

Immunogen

The immunogen for this antibody is: C-QEQHVDSQNKNDK

Target

Prdm9

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Mouse

Tested Applications

ELISA

NCBI Accession Number

NP_659058.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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