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CYBRD1 Antibody

CYBRD1 Antibody

Product Specifications

Product Name Alternative

CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3

UniProt

Q53TN4

Reactivity

Human

Immunogen

The immunogen for this antibody is: CRNLALDEAGQRSTM

Target

CYBRD1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1μg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567.Immunohistochemistry:In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5μg/mL. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_079119.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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