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Transcription factor E2F4 Antibody

Transcription factor E2F4 Antibody

Product Specifications

Product Name Alternative

E2F4, E2F transcription factor 4, p107/p130-binding, E2F-4, p107/p130-binding protein, transcription factor E2F4

UniProt

Q16254

Reactivity

Human, Porcine

Immunogen

The immunogen for this antibody is: C-PKELSEIFDPTR

Target

Transcription factor E2F4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 50kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0kDa according to NP_001941.2) . Recommended concentration: 0.1-0.3μg/mL. Approx 40+50kDa bands observed in Pig Brain and Heart lysates (calculated MW of 43.2kDa according to Pig XP_003126981.3) . Recommended concentration: 0.01-0.003μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001941.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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