Hoechst 34580

Hoechst 34580 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].

Product Specifications

CAS Number

[23555-00-2]

Product Name Alternative

HOE 34580

UNSPSC

12171500

Hazard Statement

H302

Target

Amyloid-β

Type

Dye Reagents

Related Pathways

Neuronal Signaling

Applications

Neuroscience-Neurodegeneration

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Hoechst-34580.html

Purity

99.84

Solubility

DMSO : ≥ 47 mg/mL

Smiles

CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(N(C)C)C=C6)N5)=C4)N3)=C2)CC1

Molecular Formula

C27H29N7

Molecular Weight

451.57

Precautions

H302

References & Citations

[1]Thai NQ, et al. Discovery of DNA dyes Hoechst 34580 and 33342 as good candidates for inhibiting amyloid beta formation: in silico and in vitro study. J Comput Aided Mol Des. 2016 Aug;30 (8) :639-50.

Shipping Conditions

Room Temperature

Storage Conditions

4°C (Powder, protect from light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Citation 01

Free Radic Biol Med. 2024 Sep 17:S0891-5849 (24) 00666-X.|J Lipid Res. 2023 Mar;64 (3) :100338.|Medical University of Vienna. 2025.|Microbiol Res. 2025 Jun 16:299:128259.|PLoS One. 2019 Mar 18;14 (3) :e0213794.