Thrombospondin Antibody

Product Specifications

Product Name Alternative

THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180

UniProt

P07996

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: NRIPESGGDNSVFD-C

Target

Thrombospondin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

NCBI Accession Number

NP_003237.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.