Pro-EGF Antibody
Pro-EGF Antibody
Product Specifications
Product Name Alternative
EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor
UniProt
P01133
Reactivity
Human
Immunogen
The immunogen for this antibody is: SRQERVCNIEKNVS
Target
Pro-EGF
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 160kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 160kDa double band observed in Human Prostate lysates (calculated MW of 134kDa according to NP_001954.2) . The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 5μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001954.2, NP_001171601.1, NP_001171602.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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