BMI1 Antibody
BMI1 Antibody
Product Specifications
Product Name Alternative
BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, polycomb complex protein BMI-1, polycomb group RING finger protein 4, polycomb
UniProt
P35226
Reactivity
Human, Mouse, Porcine
Immunogen
The immunogen for this antibody is: C-SHQRDGLTNAGELES
Target
BMI1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 40kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4) . This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20 (15) :2110-20. PMID: 16882984) . Recommended concentration: 0.1-0.3μg/mL. Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1) . Recommended concentration: 1-3μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, WB
NCBI Accession Number
NP_005171.4
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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