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BMI1 Antibody

BMI1 Antibody

Product Specifications

Product Name Alternative

BMI1, BMI1 polycomb ring finger oncogene, FLVI2/BMI1, PCGF4, RNF51, B lymphoma Mo-MLV insertion region 1 homolog, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, polycomb complex protein BMI-1, polycomb group RING finger protein 4, polycomb

UniProt

P35226

Reactivity

Human, Mouse, Porcine

Immunogen

The immunogen for this antibody is: C-SHQRDGLTNAGELES

Target

BMI1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 40kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4) . This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20 (15) :2110-20. PMID: 16882984) . Recommended concentration: 0.1-0.3μg/mL. Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1) . Recommended concentration: 1-3μg/mL.

Prediction Reactivity

Canine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_005171.4

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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