POMC Antibody

Product Specifications

Product Name Alternative

POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating, hormone/ beta-endorphin, LPH, NPP, OTTHUMP00000119991, OTTHUMP00000200964, adrenocorticotropic hormone, adrenocorticotropin, alpha-MSH, alpha-melanocyte-stimulating hormone, beta-LPH, beta-endorphin, beta-melanocyte-stimulating hormone, corticotropin-like intermediary peptide, gamma-LPH, gamma-MSH, lipotropin beta, lipotropin gamma, melanotropin alpha, melanotropin beta, melanotropin gamma, met-enkephalin, pro-ACTH-endorphin, proopiomelanocortin preproprotein

UniProt

P01189

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-NAIIKNAYKKGE

Target

POMC

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 27kDa band observed in lysates of cell line NIH3T3 and approx 30kDa in Rat Brain lysates (calculated MW of 26.7kDa according to Mouse NP_001265510.1 1 and 26.6kDa according to Rat NP_647542.1) . Recommended concentration: 0.3-2μg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Rat

Tested Applications

ELISA, FC, IHC, WB

NCBI Accession Number

NP_000930.1, NP_001030333.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.