CD3E Antibody
CD3E Antibody
Product Specifications
Product Name Alternative
CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain
UniProt
P07766
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RKGQRDLYSGLNQR
Target
CD3E
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 22kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1) . Recommended concentration: 0.1-0.3μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Porcine, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_000724.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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