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CAPRIN1 Antibody

CAPRIN1 Antibody

Product Specifications

Product Name Alternative

CAPRIN1, cell cycle associated protein 1, GPIAP1, GPIP137, M11S1, RNG105, p137GPI, GPI-anchored membrane protein 1, GPI-anchored protein p137, GPI-p137, RNA granule protein 105, activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle

UniProt

Q14444

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-EQRPQKEPIDQ

Target

CAPRIN1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 80 + 140kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80+140kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4kDa according to NP_005889.3) . The ~140kDa band corresponds to the reported p137 (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270 (35) :20717-23.PMID: 7657653) . Recommended concentration: 0.3-1μg/mL.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 5μg/mL.

Prediction Reactivity

Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_976240.1, NP_005889.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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