Vimentin Antibody
Vimentin Antibody
Product Specifications
Product Name Alternative
VIM, vimentin, FLJ36605
UniProt
P08670
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-QVINETSQHHDDLE
Target
Vimentin
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 55kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1) . Recommended concentration: 0.1-2μg/mL.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5μg/mL. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, FC, IF, WB
NCBI Accession Number
NP_003371.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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