AIFM1 Antibody
AIFM1 Antibody
Product Specifications
Product Name Alternative
AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
UniProt
O95831
Reactivity
Human, Mouse, Porcine, Rat
Immunogen
The immunogen for this antibody is: C-NEVAKLFNIHED
Target
AIFM1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 70kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1) . Recommended concentration: 0.01-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_004199.1, NP_001124318.1, NP_665811.1, NP_665812.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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