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AIFM1 Antibody

AIFM1 Antibody

Product Specifications

Product Name Alternative

AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor

UniProt

O95831

Reactivity

Human, Mouse, Porcine, Rat

Immunogen

The immunogen for this antibody is: C-NEVAKLFNIHED

Target

AIFM1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 70kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1) . Recommended concentration: 0.01-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_004199.1, NP_001124318.1, NP_665811.1, NP_665812.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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