APEX1 Antibody

APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA- (apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic), APEX nuclease 1 endonuclease, deoxyribonuclease (apurinic or apyrimidinic), apurinic/apyrimidinic (abasic) endonuclease, redox factor 1

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1) . Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.