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CDYL Antibody

CDYL Antibody

Product Specifications

Product Name Alternative

CDYL, chromodomain protein, Y-like, CDYL1, DKFZP586C1622, MGC131936, CDY-like, autosomal, bA620A17.2 (chromodomain protein, Y chromosome-like), chromodomain protein, Y chromosome-like, chromodomain protein, Y chromosome-like, isoform b, testis-specific chromodomain Y-like protein, DKFZp586C1622

UniProt

Q9Y232

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SMLKYLQRKIDEF

Target

CDYL

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99 (13) :8707-12; PMID: 12072557) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001137442.1, NP_001137443.1, NP_004815.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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