DDB1 Antibody
DDB1 Antibody
Product Specifications
Product Name Alternative
DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE
UniProt
Q16531
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-DLIKVVEELTRIH
Target
DDB1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9kDa according to Human NP_001914.3) . Recommended concentration: 1-2μg/mL. Approx 140kDa band observed in lysates of cell line NIH3T3 and approx 150kDa band observed in lysates of cell line NSO (calculated MW of 126.8kDa according to Mouse NP_056550.1) . Recommended concentration: 0.01-0.03μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_001914.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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