FOXP3 Antibody
FOXP3 Antibody
Product Specifications
Product Name Alternative
FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin
UniProt
Q9BZS1
Reactivity
Human
Immunogen
The immunogen for this antibody is: SQRPSRCSNPTPGP
Target
FOXP3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10μg/mL. Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4μg/mL for overnight staining.
Tested Applications
ELISA, FC, ICC, IF, WB
NCBI Accession Number
NP_054728.2, NP_001107849.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
