FXR1 Antibody

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1) . The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7 (13) :2121-8.; PMID: 9817930) . An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.